Our Service Includes:
- Obtaining and sub-cloning gene into either our own optimized proprietary expression vector or into an expression vector of your choice
- Sequence verification
- DNA preparation and quantitation
- Site-directed mutagenesis if needed
- Stable transfection into cell line of your choice
- Co-transfection of Gα16, reporter gene or any other necessary or requested component
- Cell selection (G418, hygromycin, puromycin, etc )
- Colony isolation & screening by
- Intracellular calcium (FexStation Plate Reader)
- Intracellular cAMP determinations
- Radioligand binding
- ELISA, Western
- Identify, validate and cryopreserve best and multiple back-up colonies
- Bank best colony
- Test for Mycoplasma prior to shipping
- Send weekly updates and comprehensive final report
Prior to initiating a project, we will ask you for your specific requirements, then will tailor a proposal that meets those requirements. Of course, this is typically done under terms of a confidentiality agreement.
Applied Cell Sciences has experience with GPCRs, kinases (RTKs), promiscuous G proteins, ion channels, reporter genes, and transporters and has constructed and validated over 70 stable, high-expression, pharmacologically validated cell lines for clients.
Ordering Information:
To discuss requirements, timing and pricing
please call 240-453-6331 or email inquiries@appliedcellsci.com.